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MicroRNA Protocols

Methods in Molecular Biology 342

Erschienen am 01.04.2006, 1. Auflage 2006
160,49 €
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In den Warenkorb
Bibliografische Daten
ISBN/EAN: 9781588295811
Sprache: Englisch
Umfang: xiv, 366 S., 80 s/w Illustr., 3 farbige Illustr.,
Einband: gebundenes Buch

Beschreibung

InhaltsangabeThe MicroRNA: Overview of the RNA Gene That Modulates Gene Functions ShaoYao Ying, Donald C. Chang, Joseph D. Miller, and ShiLung Lin Structure Analysis of MicroRNA Precursors Jacek Krol and Wlodzimierz J. Krzyzosiak MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex Richard I. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhattar Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery Stefan A. Muljo and Chryssa Kanellopoulou MicroRNAs and Messenger RNA Turnover Julia A. Chekanova and Dmitry A. Belostotsky Prediction of MicroRNA Targets Marc Rehmsmeier Prediction of Human MicroRNA Targets Bino John, Chris Sander, and Debora S. Marks Complications in Mammalian MicroRNA Target Prediction Neil R. Smalheiser and Vetle I. Torvik miRBase:The MicroRNA Sequence Database Sam Griffiths-Jones Methodologies for High-Throughput Expression Profiling of MicroRNAs Paz Einat In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development Catherine Kidner and Marja Timmermans Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA Fengfeng Zhuang and Yi-Hsin Liu Cloning MicroRNAs From Mammalian Tissues Michael Z. Michael Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation Hyeyoung Min and Chang-Zheng Chen Identifying MicroRNA Regulators of Cell Death in Drosophila ChunHong Chen, Ming Guo, and Bruce A. Hay MicroRNAs in Human Immunodeficiency Virus-1 Infection Yamina Bennasser, Shu-Yun Le, Man Lung Yeung, and Kuan-Teh Jeang Cloning and Detection of HIV-1-EncodedMicroRNA Shinya Omoto and Yoichi R. Fujii Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein Ranhui Duan and Peng Jin In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates Akira Ishizuka, Kuniaki Saito, Mikiko C. Siomi, and Haruhiko Siomi Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector Feng Luo, Jiing-Kuan Yee, Sheng-He Huang, Ling-Tao Wu, and Ambrose Y. Jong Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs ShiLung Lin and ShaoYao Ying Isolation and Identification of Gene-Specific MicroRNAs ShiLung Lin, Donald C. Chang, and ShaoYao Ying TransgeneLike Animal Models Using Intronic MicroRNAs ShiLung Lin, ShinJu E. Chang, and ShaoYao Ying Evolution of MicroRNAs Andrea Tanzer and Peter F. Stadler Perspectives ShaoYao Ying, Donald C. Chang, Joseph D. Miller, and ShiLung Lin Index

Autorenportrait

InhaltsangabeThe MicroRNA: Overview of the RNA Gene That Modulates Gene Functions ShaoYao Ying, Donald C. Chang, Joseph D. Miller, and ShiLung Lin Structure Analysis of MicroRNA Precursors Jacek Krol and Wlodzimierz J. Krzyzosiak MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex Richard I. Gregory, Thimmaiah P. Chendrimada, and Ramin Shiekhattar Recognition and Cleavage of Primary MicroRNA Transcripts Yan Zeng and Bryan R. Cullen Mouse Embryonic Stem Cells as a Model Genetic System to Dissect and Exploit the RNA Interference Machinery Stefan A. Muljo and Chryssa Kanellopoulou MicroRNAs and Messenger RNA Turnover Julia A. Chekanova and Dmitry A. Belostotsky Prediction of MicroRNA Targets Marc Rehmsmeier Prediction of Human MicroRNA Targets Bino John, Chris Sander, and Debora S. Marks Complications in Mammalian MicroRNA Target Prediction Neil R. Smalheiser and Vetle I. Torvik miRBase:The MicroRNA Sequence Database Sam Griffiths-Jones Methodologies for High-Throughput Expression Profiling of MicroRNAs Paz Einat In Situ Hybridization as a Tool to Study the Role of MicroRNAs in Plant Development Catherine Kidner and Marja Timmermans Usefulness of the Luciferase Reporter System to Test the Efficacy of siRNA Fengfeng Zhuang and Yi-Hsin Liu Cloning MicroRNAs From Mammalian Tissues Michael Z. Michael Methods for Analyzing MicroRNA Expression and Function During Hematopoietic Lineage Differentiation Hyeyoung Min and Chang-Zheng Chen Identifying MicroRNA Regulators of Cell Death in Drosophila ChunHong Chen, Ming Guo, and Bruce A. Hay MicroRNAs in Human Immunodeficiency Virus-1 Infection Yamina Bennasser, Shu-Yun Le, Man Lung Yeung, and Kuan-Teh Jeang Cloning and Detection of HIV-1-EncodedMicroRNA Shinya Omoto and Yoichi R. Fujii Identification of Messenger RNAs and MicroRNAs Associated With Fragile X Mental Retardation Protein Ranhui Duan and Peng Jin In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates Akira Ishizuka, Kuniaki Saito, Mikiko C. Siomi, and Haruhiko Siomi Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector Feng Luo, Jiing-Kuan Yee, Sheng-He Huang, Ling-Tao Wu, and Ambrose Y. Jong Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs ShiLung Lin and ShaoYao Ying Isolation and Identification of Gene-Specific MicroRNAs ShiLung Lin, Donald C. Chang, and ShaoYao Ying TransgeneLike Animal Models Using Intronic MicroRNAs ShiLung Lin, ShinJu E. Chang, and ShaoYao Ying Evolution of MicroRNAs Andrea Tanzer and Peter F. Stadler Perspectives ShaoYao Ying, Donald C. Chang, Joseph D. Miller, and ShiLung Lin Index

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